Simple, rapid, precise, accurate and novel orthogonal function spectrophotometry (OFS) in combination with zero order UV-spectrophotometry was developed and validated for simultaneous estimation of Lornoxicam (LOR) and Tolperisone Hydrochloride (TPH) in tablet dosage form. This method involves estimation of TPH by six point quadratic orthogonal polynomials function with equally spaced intervals at 240, 245, 250, 255, 260 and 265 nm which eliminate interference of LOR and estimation of LOR at 385 nm in zero order spectra where TPH shows no characteristic absorption. Developed method was validated according to ICH guidelines. The calibration graph was found to be linear in the range of 4.0-14.0 μg/ml for both LOR and TPH with coefficient of determination (r2) value greater than 0.999. Accuracy of method was confirmed by recovery studies showing recovery between 98% to 102%. Reproducibility and repeatability was confirmed by intraday and interday precision where % RSD was found to be less than 2. The method was successfully applied for estimation of LOR and TPH in tablet dosage form.
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